Difference Between Flash Column And Conventional Column
The column chromatography is a kind of separation and analysis technology, it can use the liquid as the mobile phase, and paper, sheet and packed bed as the stationary phase. During the development process of chromatography technology, in order to distinguish various methods, we name them according to different operating process, such as flash column chromatography and conventional column chromatography.
Specifically, the conventional column chromatography always uses a porous stationary phase with coarse particle, and then fill the phase in the long glass column with heavy caliber. The mobile phase flows through the chromatography column only by gravity.
The mass transfer and diffusion rate of the solute in the stationary phase are slow. Moreover, the inlet pressure of the column is low and the efficiency is poor, so the analysis time is relevantly lengthy.
On the other side, the flash column chromatography uses a macro-reticular stationary phase with fine particle, and it is filled in the short stainless-steel column with minor caliber. The mobile phase flows through the chromatography column with high column pressure by the high-pressure pump.
The mass transfer and diffusion rate of the solute in the stationary phase are much faster, so that we can achieve high column efficiency and high separation capacity in short analysis time.