Flash column is used for preparation and has the following advantages:
1. Fast speed
Generally 1/8 of the separation time of the open column chromatography, saving time, slow decomposition of sample changes.
2. Large preparation amount
Compared with HPLC preparation column of the same diameter, at least twice as many samples can be obtained
3. Low cost
First, it is lower than HPLC; secondly, the solvent is saved compared with the glass column, and the silica gel column can be used multiple times, saving the amount of silica gel.
No special solvent treatment-samples can be loaded dry, no special training for operators.
The effect of the flash column depends mainly on the optimization of the elution solvent and the column loading effect. In the relationship between processing and preparing with HPLC, the following principles should be followed:
(1) When the flash column can be used, no HPLC is required;
(2) First, use the flash column, and then use HPLC to purify.
Other tips are:
1. First, use TLC to select the antisolvent and load
2. Use short and thick columns (diameter: length <=1:7)
Hawach flash column
Hawach has three series of flash columns:
StarFlash series: only silica gel column, divided into standard and advanced, advanced silica gel with higher purity and better quality.
DepuFlash series: Corresponding to silica gel and phase column, C18 (spherical and irregular), C8, NH2, CN, Phenyl, SAX, SCX, Diol, which are made for the general market.
Other Flash series: Corresponding to silica gel construction and phase column, C18 (spherical inlet and irregular domestic), C4, C8, NH2, CN, HILIC, aluminum oxide, etc. which are made for the high-end market.
The materials used are all medical grade PP, PE, with strong corrosion resistance and low impurity content.