Physical Properties of Flash Column
The high surface area has strong retention, column capacity, and separation degree for the separation of multicomponent samples. Fillers with low surface area usually reach equilibrium quickly, which is particularly important for gradient eluting. The smaller the average particle size, the smaller the particle distribution, the higher the chromatographic column efficiency, and the higher the backpressure.
Availability of Flash Column
When the basic molecule (pyridine) was analyzed at high pH, the column effect, peak trailing, and retention difference could be observed. This indicates that there is still an unwelcome secondary interaction between the basic molecules and the silicone groups on the surface of the stationary phase. After medium pH comparison, when alkaline molecules (pyridine) were analyzed at high pH, the Hawach flash column once again provided excellent peak shape and column effect, indicating that the unpopular secondary interaction had been almost eliminated. These adverse interactions may also lead to poor reproducibility of chromatographic columns. Changing pH is a powerful tool to optimize selectivity and identify sample impurities.
Specification of Flash Column
Hawach flash columns are designed to provide excellent peak shape and life in LC/MS compatible acidic, neutral, and alkaline pH buffers, and can be used in conjunction with mobile phase containing methanol or acetonitrile without reducing column efficiency or prolonging equilibrium time. The super lazy flash columns with extended pH stability for UHPLC and HPLC selective LC/MS compatible buffers between pH 1.5 and 11.5 at low, medium, and high pH conditions, and the best performance and reproducibility can be obtained.
A typical flash column for chromatography is made of stainless steel or glass, with a length of 10-30 cm and a diameter of 2.5-7 cm. It contains a stationary phase with 30-50 μm particle size, usually silica gel. The column operates at low pressures (<100 psi) and high flow rates. It must be compatible with the solvents used and have standard connectors for chromatography systems. The column should also be chemically resistant and have end caps to prevent leakage. Always follow manufacturer guidelines for packing and use.