Beneficial Effects and Device Setup of Flash column
Flash is a rapid separation mode for preparative chromatography, using optimized pre-loaded medium and low-pressure columns for chromatographic separation. Flash is considered a medium to low-pressure separation chromatography, and the resolution is correspondingly lower than HPLC (high-performance liquid chromatography, or HPLC-HPLC). Flash technology, developed in 1978 to purify organic compounds, is fast and inexpensive compared to conventional column chromatography. Now, Flash separation and purification technology are widely used in drug research and development, sample purification and purification of natural products and other applications. Because of its low cost and simplicity, Flash is one of the chromatographic separation and purification tools that cannot be replaced by HPLC.
General specification of Flash column
The most commonly used packing for Flash was silica gel because: at that time, the choice of packing for chromatographic column and TLC was limited; Other bonds and phases like C8 and C18 are too expensive.
Therefore, most of the Flash application methods available in the literature are developed with silica gel as the separation matrix. Flash is basically the normal phase liquid chromatography separation technology. Also, have the use of reverse-phase silica gel filler, But most users prefer Flash technology to use silicone. Usually, the size of the sample is milligram-100g. The flow rate ranged from 10mL/min to 300mL/min. More use of low – polarity organic solvents as mobile phase such as hexane, ethyl acetate.
Asimo rapid separation column adopts the proprietary technology of automatic loading, the column bed is tight and uniform, and there is no “ditch flow effect”.The high degree of separation, good reproducibility.
The positive and reverse-phase silica gel fillers with different particle sizes (minimum 15 m) allow users to freely choose different fast separation columns in terms of separation degree, sample loading quantity and flow rate according to different demands of economy and efficiency.
The rapid separation column is made of medical-grade polypropylene (PP) material, which avoids the dissolution pollution of PP material to the separated sample. In addition, the transparent PP material column can also visually observe the separation of samples. The patented cylinder design enables the rapid separation column to withstand up to 300psi without leakage.
The rapid column chromatography separation system can greatly improve the separation efficiency of reaction products. Generally speaking, column chromatography takes 5~6 hours at a time, sometimes more than 10 hours. The instrument can separate the sample in a few minutes with higher purity. This can save a lot of solvent and chromatography silica gel, but also can save a lot of time.
Separation column, used in conjunction with a small separation column, the product is especially suitable for positive screening. Separation column device: used in conjunction with a small column to separate cells, the product is especially suitable for positive screening.
The column is the main part of the gel chromatography technique. According to the different distribution coefficients of each component in the sample mixture in the fixed phase and the mobile phase, a chromatography method for separation is widely used in the fields of chemistry, chemical engineering, and medicine. However, the traditional chromatography column separation speed is slow, the experimental period is too long, resulting in a great waste of manpower and resources.
In order to solve the above problems, a fast separation column with negative pressure is proposed, which can extract the space between the conical receiver and the lower part of the column body into negative pressure by vacuum pump, thus improving the reagent velocity in the column and saving the separation time.
In order to solve the technical problems, the technical scheme is as follows: a negative pressure rapid separation chromatography column, including the column body, the top of the column body transverse bending and a plug; The center of the column body is provided with an expanded spherical column body, the lower diameter is reduced and a valve is provided; A conical receiving bottle is arranged under the cylinder body, and the cylinder body is connected with the conical receiving bottle by a joint.
The joint is of the double glass structure, the bottom end of the column body is inserted into its inner diameter, the top of the interlayer of the joint is sealed, the bottom end is open and connected with the conical receiver bottle, and the sidewall of the interlayer of the joint is connected with a recovery bottle by a vacuum catheter. The recovery bottle is also equipped with a vacuum pump connection tube to connect the vacuum pump.
The bottom part of the joint is provided with internal thread, and the top port of the conical receiver is provided with thread.
The sidewall of the joint is provided with an outlet for connecting the vacuum conduit.
Threads are arranged on the outlet and the vacuum conduit respectively.
The top part of the recovery bottle is provided with a seal plug, the vacuum catheter and the vacuum pump connection pipe are inserted into the recovery bottle through the sealing plug.
The invention relates to a fast negative pressure separation chromatography column, which can extract the space between the conical receiver bottle and the lower part of the column body as negative pressure through a vacuum pump, so as to improve the reagent flow rate in the column and save the separation time.
1. Cylinder body,
3. Conical receiver bottle,
5. Vacuum catheter,
6. Recovery bottle,
7. Vacuum pump connection pipe.
Negative pressure rapid separation chromatography column, including column body 1, column body I top transverse bending with plug 2. The cylinder body I am provided with an expanded spherical cylinder body in the middle, and its lower diameter is reduced and equipped with a valve. A conical receiver bottle 3 is arranged below the cylinder body I, and the cylinder body I am connected with the conical receiver bottle 3 by a sub 4. Joint 4 is a double-layer glass structure, the bottom end of column body I am inserted into its inner diameter, the top of the sandwich of the said joint 4 is sealed, the bottom end is open and is connected with the conical receiver bottle 3, and the sidewall of the sandwich of the joint 4 is connected with a recovery bottle 6 by vacuum catheter 5. The recovery bottle 6 is also equipped with a vacuum pump connection tube 7 to connect the vacuum pump. The bottom port of joint 4 is provided with internal thread, and the top port of conical receiver 3 is provided with thread.