Difference Between Flash Column And Conventional Column
Flash chromatography vs column chromatography. Column chromatography is a kind of separation and analysis technology, it can use the liquid as the mobile phase, and paper, sheet, and packed bed as the stationary phase. During the development process of chromatography technology, in order to distinguish various methods, we name them according to different operating processes, such as flash column chromatography and conventional column chromatography. Hawach provided hot-selling empty flash column chromatography, spherical SAX flash column chromatography, and superior silica flash column chromatography for your choice, for other columns, please click all products.
Specifically, the conventional column chromatography always uses a porous stationary phase with coarse particle, and then fill the phase in the long glass column with heavy caliber. The mobile phase flows through the flash chromatography only by gravity. The mass transfer and diffusion rate of the solute in the stationary phase are slow. Moreover, the inlet pressure of the column is low and the efficiency is poor, so the analysis time is relevantly lengthy.
On the other side, the flash column chromatography uses a macro-reticular stationary phase with fine particles, and it is filled in the short stainless-steel column with minor caliber. The mobile phase flows through the chromatography column with high column pressure by the high-pressure pump. The mass transfer and diffusion rate of the solute in the stationary phase are much faster, so we can achieve high column efficiency and high separation capacity in a short analysis time.
Compared with traditional column chromatography, it has disadvantages such as inefficiency, complexity, trouble, and great harm. Clark Still proposed the concept of Flash chromatography (FC) in 1978, that is, rapid preparative chromatography, which is different from traditional gravity-driven column chromatography. In comparison, the characteristics of Flash chromatography are (1) Using silica gel with a slightly smaller particle size as a filler (40-63 μm), the column efficiency is high; (2) Using compressed air to apply pressure to the solvent in the column (about 10-15 psi), the separation speed is fast.