Hawach C8 Flash Columns, Always Ready for Your Separation Requirements

Described as a “quick-and-dirty” method in literature, flash chromatography is a method of liquid chromatographic separation in chemical and organic laboratories, especially when you seek fast preparative applications. flash chromatography can be carried out with hands or with the appropriate equipment.

Flash column chromatography has become a common tool when separating fine chemicals since its description in the 1970s. Different from HPLC, in process of the flash chromatography, compounds will be separated on normal phases under low pressure. Therefore, flash chromatography is called low-pressure or medium-pressure liquid chromatography.

If you compare flash chromatography with HPLC separation, you will find that flash chromatography is more efficient and economical, in the way of saving both of your time and money. Different from HPLC, in process of the flash chromatography, compounds will be separated on normal phases under low pressure. Therefore, flash chromatography is called low-pressure or medium-pressure liquid chromatography.

A flash chromatography system is perfect for precisely analyzing the separation peak by using a UV or IR detector. Due to the modular design, the flash column chromatography systems can be reconfigured too.

empty flash column chromatography

Twenty years ago, the first packed flash chromatography columns were marked as a big progress in the field of flash chromatography. Today, the legend is still helping us with a whole new level of improving the quality and efficiency of the process of isolating a single chemical compound from mixed samples.

Packed with C8 reversed phase silica, Hawach C8 columns have an excellent resolution for critical separations. Compared with C18 columns, they can not only provide faster analysis but increase selectivity and reproducing ability as well. Hawach C8 columns which need a marginally more polar phase than C18, have a high surface area. That factor can provide additional resolution to the polar phase.

As a kind of less hydrophobic column with high surface area, optimal carbon load, and unique bonded silica packing, the Hawach C8 column is an ideal choice when analytical laboratories need extra selectivity and higher speed than the C18 column. These columns also can provide you stable and reliable results with a long using-life, high silica gel quality, worldwide Luer fittings, and the lowest cost for your lab.

As of my last knowledge update in January 2022, I don’t have specific information about the “Hawach C8 Flash Columns.” However, I can provide you with a general overview of C8 flash columns and their common use in chromatography.

C8 flash columns typically refer to chromatography columns packed with a stationary phase that contains octyl (C8) groups. These columns are commonly used in reversed-phase chromatography, a technique widely applied in the separation and purification of organic compounds.

Here is a general description of what you might expect from C8 flash columns:

Features of C8 Flash Columns:

  1. Stationary Phase:
    • The stationary phase in C8 flash columns is composed of C8 alkyl chains bonded to the silica support. This hydrophobic phase is used for separating nonpolar and moderately polar compounds.
  2. Application in Reversed-Phase Chromatography:
    • C8 columns are typically employed in reversed-phase chromatography, where the stationary phase is more nonpolar than the mobile phase. This allows for the separation of compounds based on their hydrophobicity.
  3. Sample Types:
    • C8 columns are suitable for separating a wide range of organic compounds, including pharmaceuticals, natural products, and other organic molecules.
  4. Separation Mechanism:
    • The separation is based on the differential affinity of analytes for the hydrophobic C8 stationary phase. Nonpolar compounds are retained longer, while polar compounds elute more quickly.
  5. Flash Chromatography:
    • Flash chromatography is a high-speed form of preparative column chromatography. It is often used for the rapid separation and purification of compounds in organic synthesis.
  6. Hydrophobicity:
    • C8 flash columns exhibit moderate hydrophobicity, allowing for the separation of compounds based on their hydrophobic interactions with the stationary phase.
  7. Selectivity:
    • C8 stationary phase provides selectivity in the separation of compounds, particularly those that may not be well resolved on more polar stationary phases.
  8. Reversed-Phase Chromatography:
    • C8 flash columns are often used in reversed-phase flash chromatography, where non-polar mobile phases (organic solvents) are used to elute compounds.
  9. Compatibility with Organic Solvents:
    • C8 columns are compatible with a variety of organic solvents commonly used in flash chromatography, such as methanol, acetonitrile, and mixtures of these solvents.

Distribution Balance of Flash Column

Hawach flash column is a distribution method based on distribution balance. The chromatographic system consists of two phases, one is the stationary phase and the other is the mobile phase. When the two phases move relatively, the difference in the distribution equilibrium properties of each component in the mixture is repeatedly used, and finally, the purpose of separating each other is achieved.

Chromatography has been invented for more than 80 years. It is a common method for purifying and separating organic or inorganic substances. The stationary phase polarity is larger than the mobile phase, and the opposite is reverse phase chromatography.

The Activity of Adsorbent and Its Regulation

The adsorption capacity of adsorbents is often called activity. The adsorbent activity depends on how much of their water content and the most active adsorbent contains the least amount of water. The activity of adsorbent is generally divided into five levels, expressed by I、II、III、IV, and V, respectively. The larger the number IS, the smaller the activity IS. Adding certain water to the adsorbent can reduce its activity; conversely, if some water in the adsorbent is removed by heating treatment, it can increase its activity, which is called the activation of the adsorbent.