Installation and Maintenance of Flash Column

Flash column from Hawach Scientific features high compatibility, safety, and flexibility, most of them can be used for the separation of different compounds with good flow distribution. Customized packing and clean packaging make the super clean environment and better shipping cost, high purity packing material ensures excellent separation efficiency, average pore size offers excellent flow rate, fine design enlarges load capacity and high recovery rates, and tight particle-size distribution avoids leaching and channeling.

Installation of Flash Column

The flash column supplied by Hawach is convenient for manual assembly, there are some tips for installation and usage of the Flash column(such as Empty Chromatography Columns, Spherical C4 Flash Column, Spherical SAX Flash Column, and other flash columns for your choice.). The whole equipment consists of several parts, including a screw cap, frits, tips, O-ring, end tips, and disbursing unit. Luer-lock end fittings are compatible with any flash system, end fittings keep media from moisture, polyethylene frits prevent media from leaking, as well as allow the mobile phase to pass through. More details can be referred to as instructions or communicated to our office.

Notes Before using the column

Before using the Flash column, the performance of the column should be tested, and the results should be saved as a reference for future evaluation of column performance changes.

However, it should be noted that the column performance may be different due to the difference in the sample, mobile phase, column temperature, and other conditions used; in addition, the column performance test is done according to the chromatographic column factory report (the conditions used in the factory test is better). Only in this way, the measured results are comparable.

c8 chromatography flash column

Daily maintenance of Flash Column

Flash column designed by Hawach Scientific is of transparent appearance and smooth interior walls, these properties enable customers to clean the flash column easily and completely. And each part can be dismantled and separately stored, thus contaminated risk is greatly reduced.

1. Before testing, the sample and mobile phase to be filtered are suggested;
2. It is recommended to clean in time after finishing the samples every day;
3. Routine testing: After the test, directly connect the chromatographic column in the reverse direction and wash it with 90% organic phase for 45min, then store it in pure methanol or pure acetonitrile;
4. Conditions for using buffer salts
1) Isocratic conditions: before and after using the buffer salt, flush with the transition mobile phase at a flow rate of 1ml/min for 45min;
2) Gradient conditions: before using the buffer salt, the transition mobile phase with the same composition as the initial mobile phase is rinsed at a flow rate of 1 ml/min for 45 min;
3) After the buffer salt is washed, 90% organic reverse washing is used for 60 minutes, and then stored in a pure organic solvent;
Note: If the buffer is used, it cannot be left in the column overnight.

Column storage

1. Column storage in a short time
If the interval of use is not more than four days, the column does not require special maintenance and only needs to be rinsed with pure methanol, pure water, or a mixed solution of methanol and water according to the nature of the mobile phase or sample used in the subsequent test. Wash until the baseline is flat for about half an hour.

For short-term storage of a flash column, ensure it’s clean and dry. Cap both ends to prevent contamination and label with essential details. Store upright in a cool, dry place, away from direct sunlight. Regularly check for damage and avoid solvent exposure. Follow manufacturer guidelines for specific recommendations.
2. Long-term storage
If the chromatographic column is not used for a long time, remove it from the instrument and store it. Rinse with a suitable solvent before removing it to wash off the eluent that is not suitable for storing the chromatographic column. For example, spherical C18 Flash column can be used with pure methanol or Acetonitrile is used as the storage medium, and then plug it tightly with a plastic stopper to prevent the column from drying out completely.

For long-term storage of a flash column, thoroughly clean and dry it. Cap both ends securely to prevent dust and contaminants. Store in a cool, dry place, away from sunlight and extreme temperatures. Label with essential details, and consider sealing the bottom to maintain stationary phase integrity. Periodically check for damage and ensure the column remains sealed. Follow manufacturer guidelines for specific recommendations, especially if the stationary phase is sensitive to prolonged storage conditions.