General specifications of flash columns
• The most commonly used filler for Flash is silica gel (most specifications are 40-63 60Å) because:
-Limited choice of packing materials for columns and TLC at the time.
-Other bonds and phases such as C8, and C18 are too expensive.
Therefore, most of the Flash application methods available in the literature are developed using silica gel as the separation matrix.
• Flash is basically normal phase liquid chromatography separation technology. There are also reversed-phase silica fillers; however, most users prefer flash technology to use silica gel.
• The typical loading scale is from milligrams to a hundred grams. The flow rate is from 10 mL/min to 300 mL/min.
• Use organic solvents of low to medium polarity as mobile phases such as hexane, ethyl acetate, etc.
Hawach quick-separation columns are packed with proprietary technology for automatic packing, and the column bed is compact and uniform without a “ditch flow effect”. High resolution and good reproducibility.
Different particle sizes (minimum 15 μm) of normal phase and reversed-phase silica packing can allow users to freely choose different fast separation columns in terms of resolution, sample volume, and flow rate according to different needs of the economy and performance.
The rapid separation column is made of medical-grade polypropylene (PP) material, which avoids the dissolution pollution of common PP materials on the separation sample. In addition, the transparent PP material cylinder allows the user to visually observe the separation of the sample. The patented column design allows the maximum separation pressure of the rapid separation column to reach 300 psi, and it is safe and leak-free.
Fast separation columns have Luer-Lock standard fittings and can be used on a variety of fast liquid chromatography separation systems.