Reliable and Reproducible
Hawach flash columns are precision-packed for high resolution as well as reproducibility. It features a one-piece design with Luer end fittings for easy connection to diverse chromatography systems. Hawach constantly pursues top technology to improve consumer feeling, for example, automatically detect the column type and size, and programs a default method, which can greatly reduce setup time and the potential errors in operation. A special chromatography technique uses compressed gas or a pump to make solvent pass flow through the column smoothly. As opposed to conventional gravity flow, Hawach flash column allows for faster flow rates and give special consideration for the stationary phase such as silica gel in the usage of finer particle size.
Versatile and Safe
Hawach flash columns are available in extra thick walls and cartridge pressure for safe operation. Flash column are all-welded end fittings ensure the column is able to withstand the pressure stability and not leak valuable compound. A wide offering of TLC plates makes operation easy, and delicate stationary phases expand the utility of the flash column. Moreover, high-quality media is specified for high resolution and reproducibility for high standard purification.
The selection of the mobile phase
The commonly used mobile phases are water with acetonitrile or water with methanol, occasionally water with tetrahydrofuran. Generally, we can add some weak acids or weak bases to adjust the liquid systems to partial acids or alkalis, which makes sure that the components can peak.
The commonly used weak acids are formic acid, trifluoroacetic acid. And the commonly used weak bases are ammonia water, ammonia bicarbonate, etc. The percentage added is generally the same as that in liquid systems.
A sampling of reversed-phase flash columns
In general, liquid phase wet sampling is used. Commonly used solvents include water, methanol, tetrahydrofuran, acetonitrile, NH-dimethylformamide, dimethyl sulfoxide.