Reliable and Reproducible
Hawach flash columns are precision-packed for high resolution as well as reproducibility. It features a one-piece design with Luer end fittings for easy connection to diverse chromatography systems. Hawach constantly pursues top technology to improve consumer feeling, for example, automatically detects the column type and size, and programs a default method, which can greatly reduce setup time and the potential errors in operation. A special chromatography technique uses compressed gas or a pump to make solvent pass flow through the column smoothly. As opposed to conventional gravity flow, the Hawach flash column allows for faster flow rates and gives special consideration to the stationary phase such as silica gel in the usage of finer particle size.
Versatile and Safe
Hawach flash columns are available in extra thick walls and cartridge pressure for safe operation. Flash columns are all-welded end fittings that ensure the column is able to withstand pressure stability and not leak valuable compounds. A wide offering of TLC plates makes operation easy, and delicate stationary phases expand the utility of the flash column. Moreover, high-quality media is specified for high resolution and reproducibility for high-standard purification.
The selection of the mobile phase
The commonly used mobile phases are water with acetonitrile or water with methanol, and occasionally water with tetrahydrofuran. Generally, we can add some weak acids or weak bases to adjust the liquid systems to partial acids or alkalis, which makes sure that the components can peak.
The commonly used weak acids are formic acid, trifluoroacetic acid. And the commonly used weak bases are ammonia water, ammonia bicarbonate, etc. The percentage added is generally the same as that in liquid systems.
A sampling of reversed-phase flash columnsIn general, liquid-phase wet sampling is used. Commonly used solvents include water, methanol, tetrahydrofuran, acetonitrile, NH-dimethylformamide, and dimethyl sulfoxide.