Superior Silica Gel Flash Column and Protect the Flash Column
Excellent Separation and Purification Performance
Superior silica gel flash column adopts high capacity silica gel for accurate filling, which gives it very excellent separation and purification performance. The surface area of high capacity spherical silica gel is 40% higher than that of ordinary spherical silica gel, and the sample size is twice that of ordinary spherical silica gel. Meanwhile, the surface area is 40% higher than normal spherical silica gel; a higher sample size means smaller, faster, and more economical chromatographic columns used for separation; less solvent use, and higher separation.
Good Reproducibility and Medical Grade Materials
Superior silica gel flash column is automatically filled with proprietary technology, the column bed is tight and uniform, and there is no “ditch flow effect “. Positive and inverse silica gel fillers with different particle sizes allow users to freely select different fast separation columns in terms of separation degree, sample size, and flow rate according to different economic and efficiency requirements. The rapid separation column is made of medical-grade polypropylene material, which avoids the dissolution contamination of separated samples by ordinary PP materials. Additionally, transparent PP material cylinders allow users to visually observe sample separation.
How to protect the column
The key to maintaining long column life is proper sample handling before injection. You must prevent pumping compounds that are very hydrophobic/different from the polarity of the mobile phase into the column, whether from the mobile phase or the sample. In particular, the introduction of particulate impurities should be prohibited. These will eventually increase the operating pressure and is very difficult or impossible to remove.
Be sure to use a guard column, because contamination of the sample and eluent may cause an increase in column pressure and affect selectivity. For Hawach columns, we recommend using the correct type of guard column. The packing material of this column is similar to that of the chromatography column used for analysis. The guard column needs to be replaced when the column pressure increases or the column efficiency is observed to decrease.
Attention during storage:
Do not store the column in a column filled with buffer or other salt-containing eluents. Storage solvents should contain at least 20% organic solvents to prevent the growth of bacteria.
Possible causes of loss of column efficiency:
Extra peak broadening. When using columns with small diameters or short lengths, peak broadening may be more apparent. To ensure that the length and the inner diameter of the pipeline is kept small. Check the injection volume and detector to check whether the cell volume is suitable for the column volume.
The equilibration time for elution is not enough.
Incorrect column temperature.
Incorrect correction density.
An excessive elution flow rate was used. Reverse the column and use a low flow rate.