Superior Silica Gel Flash Column and Protect the Flash Column

Excellent Separation and Purification Performance

Superior silica gel flash column adopts high capacity silica gel for accurate filling, which gives it very excellent separation and purification performance. The surface area of high capacity spherical silica gel is 40% higher than that of ordinary spherical silica gel, and the sample size is twice that of ordinary spherical silica gel. Meanwhile, the surface area is 40% higher than normal spherical silica gel; a higher sample size means smaller, faster, and more economical chromatographic columns used for separation; less solvent use, and higher separation.

Good Reproducibility and Medical Grade Materials

Superior silica gel flash column is automatically filled with proprietary technology, the column bed is tight and uniform, and there is no “ditch flow effect”. Positive and inverse silica gel fillers with different particle sizes allow users to freely select different fast separation columns in terms of separation degree, sample size, and flow rate according to different economic and efficiency requirements. The rapid separation column is made of medical-grade polypropylene material, which avoids the dissolution contamination of separated samples by ordinary PP materials. Additionally, transparent PP material cylinders allow users to visually observe sample separation.

Using a Superior Silica Gel Flash Column:

  1. Column Selection:
    • Choose a flash column with high-quality silica gel. Different columns may have variations in particle size, pore size, and bonding chemistry. Select the column that best suits your purification needs.
  2. Sample Loading:
    • Load your sample onto the flash column carefully. Ensure that the sample is evenly distributed on the top of the column to achieve better separation.
  3. Mobile Phase Selection:
    • Choose an appropriate mobile phase for your separation. The mobile phase composition and gradient play a crucial role in the separation efficiency of the flash column.
  4. Column Equilibration:
    • Allow the column to equilibrate with the mobile phase before initiating the purification process. This ensures consistent separation and elution patterns.
  5. Monitoring the Fraction Collection:
    • Monitor the elution profile by collecting fractions. Adjust the flow rate or mobile phase composition if needed to optimize separation and resolution.
  6. Recovery of Fractions:
    • Collect the fractions containing the target compounds. Concentrate or evaporate the collected fractions as necessary for further analysis or use.

Chromatographic Silica Gel Flash Columns

How to protect the column

  1. Sample Pre-Treatment:
    • If your sample contains particulates or impurities, consider pre-treating it to remove or reduce contaminants before loading onto the flash column. This helps prevent clogging and extends the column life.
  2. Use of Prefilters or Guards:
    • Consider using pre-column filters or guards to protect the flash column from particulate matter in the sample. These filters can be placed at the top of the column and are replaceable.
  3. Column Loading Limits:
    • Avoid overloading the flash column with a large sample quantity. This can lead to poor separation, decreased column efficiency, and potential damage to the column.
  4. Solvent Compatibility:
    • Ensure that the solvents used in the flash chromatography process are compatible with the flash column material. Incompatible solvents may damage the column.
  5. Proper Storage:
    • Store the flash column in a dry and cool environment. Exposure to moisture can affect the silica gel’s properties and reduce the column’s efficiency.
  6. Regular Maintenance:
    • Perform regular maintenance by flushing the column with appropriate solvents to remove any residual compounds and impurities. Follow the manufacturer’s recommendations for column care.

The key to maintaining long column life is proper sample handling before injection. You must prevent pumping compounds that are very hydrophobic/different from the polarity of the mobile phase into the column, whether from the mobile phase or the sample. In particular, the introduction of particulate impurities should be prohibited. These will eventually increase the operating pressure and is very difficult or impossible to remove.

Be sure to use a guard column, because contamination of the sample and eluent may cause an increase in column pressure and affect selectivity. For Hawach columns, we recommend using the correct type of guard column. The packing material of this column is similar to that of the chromatography column used for analysis. The guard column needs to be replaced when the column pressure increases or the column efficiency is observed to decrease.

Attention during storage

Do not store the column in a column filled with buffer or other salt-containing eluents. Storage solvents should contain at least 20% organic solvents to prevent the growth of bacteria.

Possible causes of loss of column efficiency

Extra peak broadening. When using columns with small diameters or short lengths, peak broadening may be more apparent. To ensure that the length and the inner diameter of the pipeline are kept small. Check the injection volume and detector to check whether the cell volume is suitable for the column volume.

The equilibration time for elution is not enough.
Incorrect column temperature.
Incorrect correction density.

An excessive elution flow rate was used. Reverse the column and use a low flow rate.