Silica Gel: One Of The Inorganic Substrate Of Flash Columns

The basic material of the stationary phase of the flash columns we called substrate is usually divided into three categories: inorganic materials, organic materials, and composite materials.

empty chromatography flash column

Silica gel is the widest material used to make a matrix in flash columns. Silica gel used in the flash columns usually has a porous structure, which has high mechanical strength and thermal stability. Its surface contains a large number of active hydroxyl groups because of the excellent pore structure and specific surface area. It plays an important role in the purification and separation of compounds in the normal phase purification system.

At the same time, the surface of silica gel can be easily modified to form other bonded stationary phases. And there are many kinds of stationary phases modified by silica gel substrate, and the effect of the separation and purification is much better than other kinds of substrates.

However, the stability of silica gel substrates is relatively poor under alkaline conditions. Not only that, but the presence of hydroxyl groups will also cause irreversible adsorption of some substances, especially alkaline substances.

The non-specific adsorption or denaturalization of the sample appears in the separation and purification of some biological molecular samples, resulting in the difference of peak deformation of sample elution in the chromatogram and the decrease of sample recovery.