The Basic Knowledge of Selecting the Most Suitable Flash Column
The most important process of the whole purification is selecting the most suitable Flash column. And now we will talk about the key content of the column: stationary phase. The stationary phase, commonly known as filler, is the most central part of chromatography. When we choose the variety of the stationary phase for the chromatography, we should define three aspects: a substrate, a bonded phase, and substrate parameters.
The substrate is the basic material of the stationary phase. There are three kinds of materials used to obtain substrates, which are inorganic materials, organic materials, and composite materials. The inorganic materials include silica gel, alumina, etc.
The organic materials are mainly polymer materials such as gelatin. And the composite materials usually refer to the materials which are combined inorganic materials and organic materials by means of hybridization, coating, and coating. Silica gel is the most widely used substrate in flash purification.
The bonded phase is used to modify the substrate or bonding the different groups, which forms different types of stationary phases.
The substrate parameters contain the shape, particle size, and pore size. For instance, the widely applied silica gel substrate is usually divided into the spherical and the amorphous. And the two types of silica gel have different particle sizes. All the parameters should be referred to according to the requirements of purification.