Tips for Using a Flash Column
In recent years, the application of HPLC in inorganic analysis has developed rapidly, and the use of flash columns has been more and more widely because of a method that can rapidly determine heavy metal elements in environmental water samples.
Matters needing the attention of flash columns
1. All joints may leak, pay attention to the toxicity and flammability of the mobile phase.
2. The mechanical properties of flash columns are stable, and they have passed extremely high-pressure tests. Opening chromatographic columns may reduce the pressure limit.
3. The packing size in the column is small. When opening the column, please operate in a well-ventilated environment.
4. Avoid using flash columns with pH values below 1.8 or above 8.0.
The experimental results
Flash columns – high-performance liquid chromatography was used for simultaneous determination of nicotine and cotinine in hair. The sample pretreatment method was simple and fast, and the baseline separation of nicotine and cotinine could be achieved within 1.0min. The analysis time was more than 85% less than that of the conventional chromatographic column, with the advantages of rapid, simple, high sensitivity and good repeatability.